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eq n methylmorpholine  (Chem Impex International)


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    Chem Impex International eq n methylmorpholine
    Eq N Methylmorpholine, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eq+n+methylmorpholine/us08871783-206-14-7?v=Chem+Impex+International
    Average 95 stars, based on 16 article reviews
    eq n methylmorpholine - by Bioz Stars, 2026-08
    95/100 stars

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    eq n methylmorpholine  (Chem Impex International)
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    Protocols for synthesis of the Fmoc-ACC fluorophore and its coupling to Rink amide resin have been described. ( a ) 20% 4-methylpiperidine/DMF; ( b ) Fmoc-protected P1 amino acid (5 eq.), HATU (5 eq.), and 2,4,6-collidine (5 eq.), DMF, 24 hr, or Fmoc-protected P2, P3, or P4 amino acid (6.5 eq.), HBTU (6.5 eq.), and <t>N-methylmorpholine</t> (13 eq.), DMF, 1 hr; ( c ) for acetyl (Ac): acetic anhydride/TEA/DMF/DCM (1:1:5:5), for N-morpholinyl ( m ): 4-morpholinylacetic acid hydrochloride (6.5 eq.), HBTU (6.5 eq.), and N-methylmorpholine (26 eq.), and for 5-methylisoxazolyl (iso): 5-methylisoxazole-3-carboxylic acid (6.5 eq.), HBTU (6.5 eq.), and N-methylmorpholine (13 eq.); ( d ) TFA/H 2 O/triisopropylsilane (95:2.5:2.5).
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    Average 95 stars, based on 1 article reviews
    eq n methylmorpholine - by Bioz Stars, 2026-08
    95/100 stars
    		  Buy from Supplier

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    Protocols for synthesis of the Fmoc-ACC fluorophore and its coupling to Rink amide resin have been described. ( a ) 20% 4-methylpiperidine/DMF; ( b ) Fmoc-protected P1 amino acid (5 eq.), HATU (5 eq.), and 2,4,6-collidine (5 eq.), DMF, 24 hr, or Fmoc-protected P2, P3, or P4 amino acid (6.5 eq.), HBTU (6.5 eq.), and N-methylmorpholine (13 eq.), DMF, 1 hr; ( c ) for acetyl (Ac): acetic anhydride/TEA/DMF/DCM (1:1:5:5), for N-morpholinyl ( m ): 4-morpholinylacetic acid hydrochloride (6.5 eq.), HBTU (6.5 eq.), and N-methylmorpholine (26 eq.), and for 5-methylisoxazolyl (iso): 5-methylisoxazole-3-carboxylic acid (6.5 eq.), HBTU (6.5 eq.), and N-methylmorpholine (13 eq.); ( d ) TFA/H 2 O/triisopropylsilane (95:2.5:2.5).

    Journal: eLife

    Article Title: Immunoproteasome functions explained by divergence in cleavage specificity and regulation

    doi: 10.7554/eLife.27364

    Figure Lengend Snippet: Protocols for synthesis of the Fmoc-ACC fluorophore and its coupling to Rink amide resin have been described. ( a ) 20% 4-methylpiperidine/DMF; ( b ) Fmoc-protected P1 amino acid (5 eq.), HATU (5 eq.), and 2,4,6-collidine (5 eq.), DMF, 24 hr, or Fmoc-protected P2, P3, or P4 amino acid (6.5 eq.), HBTU (6.5 eq.), and N-methylmorpholine (13 eq.), DMF, 1 hr; ( c ) for acetyl (Ac): acetic anhydride/TEA/DMF/DCM (1:1:5:5), for N-morpholinyl ( m ): 4-morpholinylacetic acid hydrochloride (6.5 eq.), HBTU (6.5 eq.), and N-methylmorpholine (26 eq.), and for 5-methylisoxazolyl (iso): 5-methylisoxazole-3-carboxylic acid (6.5 eq.), HBTU (6.5 eq.), and N-methylmorpholine (13 eq.); ( d ) TFA/H 2 O/triisopropylsilane (95:2.5:2.5).

    Article Snippet: For the 14-mer sequences, double couplings were performed using Fmoc-amino acid (5 eq.), HCTU (5 eq.), and N-methylmorpholine (20 eq.) with a 96-channel Syro II automated peptide synthesizer (Biotage).

    Techniques: